scheme 2 protocol for aqueous enzymatic oil extraction studies

scheme 2 protocol for aqueous enzymatic oil extraction studies

  • Optimization of Enzyme-Assisted Extraction of Flavonoids

    Corn husks are an important byproduct of the corn processing industry. Although they are a rich source of bioactive compounds, especially flavonoids, corn husks are usually disposed of or used as animal feed. In this paper, we investigate their recovery by an enzyme-assisted extraction process consisting of a pretreatment of the plant material with cellulase followed by solvent extraction with

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  • Enzymatic transesterification for biodiesel production

    solvents. Aqueous oil extraction (AOE) consists of breaking the oil bodies mechanically in the presence of water and collecting the floating oil from the sur-face 27. The yields of oil, understandably, are generally quite poor, but not insignificant. This encouraged the development of aqueous enzymatic oil extraction

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  • J. Braz. Chem. Soc. Article Pennsylvania State University

    (Scheme 2). To compare retention times and check the enantiomeric ratio of both the (S)-and (R)-b-amino esters 5 and 8 from their respective chromatograms, the (R)-b-amino ester 8 was prepared. The aqueous layer containing the (R)-sodium carboxylate 7 (Scheme 2) from the enzymatic resolution reaction was dried under heating in vacuum. The ester 8

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  • PEER-REVIEWED ARTICLE bioresources

    hemicellulose extraction and enzymatic hydrolysis of different species is expected to be different. at 75 °C using an oil bath. After 2 h, the suspension was allowed to cool to room temperature and Scheme of hemicellulose extraction and enzymatic hydrolysis. Hemicellulose was extracted form

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  • Purifying, Detecting, and Characterizing Proteins

    A protein must be purified before its structure and the mechanism of its action can be studied. However, because proteins vary in size, charge, and water solubility, no single method can be used to isolate all proteins. To isolate one particular protein from the estimated 10,000 different proteins in a cell is a daunting task that requires methods both for separating proteins and for detecting

    Get Price
  • Derivatization and Sample Prep for (Small) Molecules

    Extraction Partitioning in one of two liquid phases Glass ware Types of Separation Technologies for Molecules An immiscible solvent is added to the sample which then separates into 2 distinct liquid phases. Some sample analytes will go into the bottom phase (Aqueous), some will separate into the top phase (Organic) "Trizol"– a form of

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  • Applications and opportunities for ultrasound assisted

    Ultrasonic pre-treatment of the almond and apricot seeds before aqueous oil extraction and aqueous enzymatic oil extraction provided significantly higher yield with reduction in extraction time. Thus, implementation of ultrasonic pre-treatment reduced oil extraction time that may improve through put in commercial oil production process. 2.2.

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  • Enzymatic Treatment an overview ScienceDirect Topics

    3.13.3.2.2 Enzymatic Treatment. Enzymatic treatment involves the use of lytic enzymes that attack and degrade the cell wall to allow release of proteins (Middelberg, 1995 ). The use of enzymes to degrade cell wall polysaccharides is considered a gentler alternative method to alkaline or acidic approaches to protein solubilization and extraction.

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  • Aqueous enzymatic process for oil and protein extraction

    Aug 01, 2011· Five commercial enzyme mixtures were evaluated for simultaneous oil and protein extraction from Moringa seed through enzyme‐assisted aqueous extraction (EAE). Protex 7L was found to be the best enzyme for highest oil (69.4%) extraction and protein (75.4%) recovery in the aqueous phase. The quality parameters of enzyme‐assisted aqueous extracted oil (EAEO), aqueous extracted oil

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  • Development of a "Green" Aqueous Enzymatic Process to

    • Corn oil is obtained by hexane extraction and/or pressing of the oil-rich germ (embryo) portion of the corn kernel (95% from WMCG). • Corn oil is currently the # 11 edible oil in the world with an annual production of 2.5 MT (#1 soybean oil 33.6 MMT and #2 palm oil 31.4 MMT).

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  • Aqueous enzymatic sesame oil and protein extraction

    Mar 15, 2011· A higher oil recovery, from sesame seeds, in the range of 16.5–24.8% for the aqueous extraction process involving enzymes was observed compared to only 12.3% for the control (oil obtained without enzyme treatment), however, the overall recovery was still significantly (p < 0.05) lower than the hexane extraction method (50.2%) ().The amount of hexane-extracted oil (HEO) was

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  • Purifying, Detecting, and Characterizing Proteins

    A protein must be purified before its structure and the mechanism of its action can be studied. However, because proteins vary in size, charge, and water solubility, no single method can be used to isolate all proteins. To isolate one particular protein from the estimated 10,000 different proteins in a cell is a daunting task that requires methods both for separating proteins and for detecting

    Get Price
  • Aqueous and Enzymatic Extraction of Oil and Protein from

    The almond cake is a protein- and oil-rich by-product of the mechanical expression of almond oil that has the potential to be used as a source of valuable proteins and lipids for food applications. The objectives of this study were to evaluate the individual and combined effects of solids-to-liquid ratio (SLR), reaction time, and enzyme use on oil and protein extraction yields from almond cake.

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  • Multiple Modeling Techniques for Assessing Sesame Oil

    Different types of enzymes were used in aqueous solution in what is called Enzymatic-Assisted Aqueous Extraction ("EAAE"), i.e., (Perctinex Ultra SPL, Alcalase, alpha amylase, glucoamylase, pectinase, protease, lipase, and phytase) under different operating conditions. Results revealed that oil extraction quality was improved.

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  • Almond oil: A comprehensive review of chemical composition

    3.5 Enzyme-assisted aqueous extraction process. The enzyme-assisted aqueous extraction process is a promising eco-friendly alternative method to solvent extraction that enables fast and simultaneous recovery of oil and protein from most oilseeds (Rosenthal et al., 1996). This emerging technology employs enzymes that hydrolyze and break the cell

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  • Enzymatic aqueous technology for simultaneous coconut

    Several studies have been carried out on aqueous enzymatic oil extraction (Dominguez et al., 1994; Rosenthal et al., 1996). The enzymatic extraction of vegetable oils, developed at Embrapa Food Agroindustry reach the high yield extraction of tropical fruits' oil (avocado, pupunha, pequi, tucumã) when the incubation process is based on a

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  • Recent development and challenges in extraction of

    The hot compressed water extraction (HCWE) is an alternative to the screw press extraction in the SLE of palm oil and ß-carotene from palm oil mesocarp (Sharizan, Azian, Yoshiyuki, Kamal, & Yunus, 2016). HCWE is a green process that utilizes water solely as the extractant under the conditions of high pressure and high temperature.

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  • 4.3: Uses of Extraction Chemistry LibreTexts

    In order to concentrate the oil, the aqueous suspension is often extracted with a low-boiling organic solvent (Figure 4.4d), which can then be easily removed from the oil. Figure 4.4: a) Whole cloves, b) Steam distillation of cloves, c) Milky distillate composed of oil and water, d) Using extraction to separate the oil from the water.

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  • Liquid–liquid extraction

    Liquid–liquid extraction (LLE), also known as solvent extraction and partitioning, is a method to separate compounds or metal complexes, based on their relative solubilities in two different immiscible liquids, usually water (polar) and an organic solvent (non-polar). There is a net transfer of one or more species from one liquid into another liquid phase, generally from aqueous to organic.

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  • Two Approaches for CAL-B-Catalyzed Enantioselective

    Jan 12, 2021· The enzymatic hydrolysis resolution in micro-aqueous media is carried out with an optimal amount of lipase CAL-B, the conditions of 12.5 mg of lipase CAL-B in TBME/H 2 O (1/0.02) have been selected for the kinetic resolution of rac-α- phenyl alkyl esters [1a–7a] in micro-aqueous media.

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  • Aqueous Enzymatic Extraction of Oil and Protein

    An advanced aqueous extraction of soybean oil assisted by adding free oil was established in this study, which recovered 81% of the oil from soybeans with 20.73% crude oil content and produced a

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  • (PDF) A Process for the Aqueous Enzymatic Extraction of

    Scheme 1 Protocol for aqueous enzymatic oil extraction during cell culture and animal model feeding studies. The combined analytical methods developed in this work could unambiguously

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  • Extraction of wheat straw with aqueous tetra-n

    Full Article. Extraction of Wheat Straw with Aqueous Tetra-n-Butylphosphonium HydroxideUula Hyväkkö, Alistair W. T. King,* and Ilkka Kilpeläinen* The stability of tetra-n-butylphosphonium hydroxide ([P 4444][OH] (aq)) solutions and their potential for wheat straw extraction are investigated.Under certain concentration ranges, aqueous [P 4444][OH] (aq) is known to rapidly dissolve up to 20

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  • Aqueous enzyme assisted oil extraction from oilseeds

    22 focusing particularly on recent efforts undertaken to improve process economics by recovering and reusing enzymes.23 24 25 Keywords 26 aqueous oil extraction, enzyme treatment, oil yield, oil characteristics, emulsion separation 27 28 1. Introduction 29 Aqueous enzymatic extraction (AEE) is a promising method for the simultaneous 30 extraction of oil and protein from oilseeds.

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  • Aqueous and enzymatic extraction processes for the

    The yield of camellia oil was 89.37 ± 0.51%, which was significant higher (p < 0.05) than that of microwave‐assisted aqueous enzymatic extraction (MAAEE, 83.05 ± 1.14%), and was similar to

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  • A Process for the Aqueous Enzymatic Extraction of Corn Oil

    Mar 17, 2009· Aqueous Enzymatic Oil Extraction Methods (A Comparison of Schemes 1, 2, and 3) Three different processes were used to conduct the aqueous enzymatic oil extractions. Scheme 1 included both buffer and cellulase and was identical to the method used in our previous publication which utilized factory-dried corn germ from a commercial corn wet mill (1).

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  • Aqueous and enzymatic processes for edible oil extraction

    ELSEVIER Aqueous and enzymatic processes for edible oil extraction A. Rosenthal, D. L. Pyle, and K. Niranjan Department of Food Science and Technology, University of Reading, Whiteknights, Reading, United Kingdom Industrial processes for the extraction of edible oil from oilseeds generally involve a solvent extraction step which may or may not be preceded by pressing.

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  • Purifying, Detecting, and Characterizing Proteins

    A protein must be purified before its structure and the mechanism of its action can be studied. However, because proteins vary in size, charge, and water solubility, no single method can be used to isolate all proteins. To isolate one particular protein from the estimated 10,000 different proteins in a cell is a daunting task that requires methods both for separating proteins and for detecting

    Get Price
  • Improvement of Soybean Oil Solvent Extraction through

    The purpose of this study is to evaluate multienzyme hydrolysis as a pretreatment option to improve soybean oil solvent extraction and its eventual adaptation to conventional processes. Enzymatic action causes the degradation of the cell structures that contain oil. Improvements in terms of extraction, yield, and extraction rate are expected to be achieved. Soybean flakes and collets were

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  • Fractionation of lignocellulosic Nature Protocols

    This protocol requires 6–7 h for the extraction of the stabilized lignin and a basic proficiency in synthetic chemistry. Cap the vial and inject the solution onto the pH 2 aqueous

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  • Recent development and challenges in extraction of

    The hot compressed water extraction (HCWE) is an alternative to the screw press extraction in the SLE of palm oil and ß-carotene from palm oil mesocarp (Sharizan, Azian, Yoshiyuki, Kamal, & Yunus, 2016). HCWE is a green process that utilizes water solely as the extractant under the conditions of high pressure and high temperature.

    Get Price
  • A short review of green extraction technologies for rice

    Enzyme-assisted aqueous extraction. Aqueous enzymatic oil extraction is an emerging green technology . This technique facilitates the release of oil in aqueous extraction processes, while avoiding the use of organic solvents. It is worth noting that the main components of plant cell walls are cellulose, hemicellulose, pectin, lignin, and protein.

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  • Avoiding Proteolysis during the Extraction

    Prevention of proteolysis during enzyme extraction and purification typically involves the addition of protease inhibitors and possible pH adjustments and/or enzyme ligand addition to homogenization buffers (see below) to inhibit proteolysis in situ, followed by separation of contaminating proteases from the enzyme of interest by column

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  • Comparison of aqueous and enzymatic extraction combination

    Comparison of 2-step aqueous and enzymatic extraction for Zn in selected medicinal plants. Comparisons of aqueous and enzymatic extraction as a first step to characterize the elemental species present in plants are shown in Fig. 5,Fig. 6 as well as

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  • Chloroform-methanol extraction of proteins

    Protocol. To 100 µL protein sample (~100µg protein) in a 1.5mL eppendorf tube: Add 400 µL methanol and vortex thoroughly. Add 100 µL chloroform and vortex. Add 300 µL H 2 O—mixture will become cloudy with precipitate—and vortex. Centrifuge 1 minute @ 14,000g. Result is three layers: a large aqueous layer on top, a circular flake of

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  • Lab on a Chip MIT

    isolation. Target-specific enzyme labeled particles were parked and isolated in aqueous droplets using our platform. Through an enzymatic reaction, fluorescent molecules were produced and accumulated in the droplets over time, resulting in signal amplification and one order of magnitude improved sensitivity compared to a direct labeling scheme,

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  • Extraction of wheat straw with aqueous tetra-n

    Full Article. Extraction of Wheat Straw with Aqueous Tetra-n-Butylphosphonium HydroxideUula Hyväkkö, Alistair W. T. King,* and Ilkka Kilpeläinen* The stability of tetra-n-butylphosphonium hydroxide ([P 4444][OH] (aq)) solutions and their potential for wheat straw extraction are investigated.Under certain concentration ranges, aqueous [P 4444][OH] (aq) is known to rapidly dissolve up to 20

    Get Price
  • Aqueous two-phase system (ATPS): an overview and advances

    Oct 28, 2016· Aqueous two-phase system (ATPS) is a liquid-liquid fractionation technique and has gained an interest because of great potential for the extraction, separation, purification and enrichment of proteins, membranes, viruses, enzymes, nucleic acids and other biomolecules both in industry and academia. Although, the partition behavior involved in the method is complex and difficult to predict.

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  • Techniques for extraction and isolation of natural

    The low polarity of S-CO 2 makes it ideal for the extraction of non-polar natural products such as lipid and volatile oil. A modifier may be added to S-CO 2 to enhance its solvating properties significantly. Conde-Hernández extracted the essential oil of rosemary (Rosmarinus officinalis) by S-CO 2 extraction, hydro distillation and steam

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  • Solid-Phase Extraction Chemistry LibreTexts

    Solid Phase Extraction (SPE) Solid phase extraction 1 (SPE) is a sample preparation technique using a solid adsorbent contained most commonly in a cartridge device (Figure 1), or on a disk to adsorb select species from solution. SPE is used to isolate a species in a sample or to clean-up a sample before analysis. Figure 1.

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  • Protocols and troubleshooting tips Abcam

    Immunoprecipitation protocol. IP troubleshooting tips. RNA Immunoprecipitation (RIP) Using IgM antibodies for IP. UV Cross-Linking and Immunoprecipitation (CLIP) Immunostaining (19) +. BrdU immunostaining procedure for cell cultures and tissue sections. GMA (Glycol methacrylate) embedding for immmunohistochemistry protocol.

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  • Enzyme Assisted Extraction an overview ScienceDirect

    Enzyme Assisted Extraction. EAE offers remarkable merits over in practice conventional tools, i.e., (i) greater selectivity, (ii) induced efficacy, (iii) ease in extraction, (iv) safer and friendlier working conditions, (v) minimal energy requirements and usage, (vi) no or less use of harsh substances, (vii) higher end yield, (viii) no involvement of wasteful protection or deprotection stages

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  • Ultrasound-assisted aqueous enzymatic extraction of oil

    the ultrasound-assisted aqueous enzymatic extraction of oil from perilla seeds. The aim of the present study was to develop an efficient and environmental friendly method for the extraction of perilla seed oil. The effect of ultrasonic pretreatment and types of enzyme on oil

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  • A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

    Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations.

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  • An Enzymatic Bioassay for Perchlorate CLU-IN

    2. Characterize the purified PR 3. Optimize and standardize the perchlorate assay protocol using the purified PR and the chlorite bioassay 4. Determine the lower detection limits of the assay 5. Determine the interference potential of soluble cations and anions 6. Test the robustness of the assay with environmental aqueous, soil, and sediment

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  • Derivatization and Sample Prep for (Small) Molecules

    Extraction Partitioning in one of two liquid phases Glass ware Types of Separation Technologies for Molecules An immiscible solvent is added to the sample which then separates into 2 distinct liquid phases. Some sample analytes will go into the bottom phase (Aqueous), some will separate into the top phase (Organic) "Trizol"– a form of

    Get Price
  • Recent development and challenges in extraction of

    The hot compressed water extraction (HCWE) is an alternative to the screw press extraction in the SLE of palm oil and ß-carotene from palm oil mesocarp (Sharizan, Azian, Yoshiyuki, Kamal, & Yunus, 2016). HCWE is a green process that utilizes water solely as the extractant under the conditions of high pressure and high temperature.

    Get Price
  • Aqueous enzymatic extraction of coconut oil SpringerLink

    Aqueous extraction of coconut oil with various enzymes was investigated. Several enzyme preparations (cellulase, polygalacturonase, protease, and α-amylase) were used at different concentrations, pH, and temperature values to enhance oil extraction. After the oil had been released by the enzyme reaction, it was separated by centrifugation. The results showed that an enzyme

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  • (PDF) Extraction of Active Enzymes from" Hard-to-Break

    After the aqueous and oil phases were separated, usually within 2 min, the top layer of aqueous solution was moved to a new tube for subsequent process.For all the extraction protocols the cell pellets were resuspended in the appropriate extraction buffer to a concentration of 1 mg/μL.

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  • Effect of Rosehip Extraction Process on Oil and Defatted

    tablished that coconut oil obtained through enzyme-assisted extraction had lower acidity and better organoleptic properties than oil extracted by conventional methods. Bocevska et al. (6) established that FFA levels of corn oil extracted through an aqueous process, with enzymatic pretreatment, were slightly higher than in control oil samples.

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  • Multiple Modeling Techniques for Assessing Sesame Oil

    Different types of enzymes were used in aqueous solution in what is called Enzymatic-Assisted Aqueous Extraction ("EAAE"), i.e., (Perctinex Ultra SPL, Alcalase, alpha amylase, glucoamylase, pectinase, protease, lipase, and phytase) under different operating conditions. Results revealed that oil extraction quality was improved.

    Get Price
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